Device

Part:BBa_K3512012:Design

Designed by: Gourav Saha   Group: iGEM20_BITSPilani-Goa_India   (2020-10-20)


pFruB-Cra System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2652
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2490
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 170


Design Notes

The primary product of the action of the invertase enzyme is fructose and glucose. Hence the concentration of fructose inside the sugarcane stem is proportional to the activity of invertase and consequently the amount of invertase present. We employed this dynamic concentration of fructose to modulate the expression of anti-invertase.


Source

Bacterial genome and Sugarcane genome.

References